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Confocal fluorescence microscopy of POPC/Chol/C16-GlcCer GUVs with a constant POPC/Chol ratio. 3D projection images from 0.4 μm confocal slices of GUVs with POPC/Chol ratios mimicking (A) 25 and (B) 75 mol % of the lo phase and increasing molar fractions of C16-GlcCer. GUVs were labeled with NBD-DPPE and Rho-DOPE. Studies were performed at neutral and acidic pH. Scale bar, 5 μm.
