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CLSM images of HEK293T cells expressing recombinant DCC-EGFP and UNC5B-mCherry before netrin-1 stimulation. (A) Channel 1: EGFP channel showing distribution of DCC. (B) Channel 2: mCherry channel showing UNC5B distribution. (C) Composite overlay image of (A) and (B). The yellow box shows a typical ROI selected for TICCS analysis. To see this figure in color, go online.
