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Time profiles for cluster density (A) and degree of aggregation (B) of DCC-EGFP, and for the M1 interaction fraction (C) in HEK293T cells coexpressing DCC-EGFP and UNC5B-mCherry in a control experiment (dotted black line) and under netrin-1 stimulation (solid black line). Netrin-1 stimulation occurs at time frame 200, but due to the averaging nature of the analysis, the effect of netrin-1 is not exact in time. The single-frame time is 0.97 s.
