Figure 2.
Images of five freeze-dried murine fibroblasts. (a–c) X-ray dark field maps of lyophilized murine fibroblasts on a Si3N4-membrane recorded with a nanofocused beam with samples kept in a cryogenic gas stream to suppress radiation damage. Three subsequent scans (a, b, and c) are recorded, the first two with a step size of Δy,z = 1 μm, the last with Δy,z = 0.5 μm. Scale bar, 20 μm. (d) Dark field mask, defining nucleic and cytoplasmic regions for further quantification. To see this figure in color, go online.