Table 2.

Fit Results for Cryoprotected Scan Series

Scan	Region of Interest	Run	a ((photons × nm)/s)	b (1)	c (photons/s)	R2 (1)
Five cells	cytop-bckg	first	3.2 × 10−2	−4.14	1.5 × 10−1	0.9998
second	3.3 × 10−2	−4.12	1.3 × 10−1	0.9998
third	3.8 × 10−2	−4.08	1.7 × 10−1	0.9998
nuc-bckg	first	1.1 × 10−1	−4.29	3.0 × 10−1	0.9996
second	8.4 × 10−2	−4.31	2.9 × 10−1	0.9996
third	6.5 × 10−2	−4.28	3.2 × 10−1	0.9997
Three cells	cytop-bckg	first	2.5 × 10−2	−4.37	1.7 × 10−1	0.9997
second	2.2 × 10−2	−4.38	1.6 × 10−1	0.9997
nuc-bckg	first	1.0 × 10−1	−4.34	4.9 × 10−1	0.9999
second	7.9 × 10−2	−4.35	4.4 × 10−1	0.9999

cytop-bckg, cytoplasmic signal minus background signal; nuc-bckg, nucleic signal minus background signal.