Figure 3. Vc peptide induces a coil-to-helix transition in the t-SNARE C-terminus (Tc).
(A) Extension-time trajectory showing changes in SNARE folding kinetics caused by binding of the Vc peptide to the t-SNARE in the SNARE complex. The Vc-bound region is colored in cyan. The positions of different states are marked by red dashed lines and the corresponding state numbers. Data were filtered using a time window of 1 ms. (B) Close-up view of the region in A marked by a rectangle. Data were filtered using a time window of 0.6 ms. (C) Probability density distributions of the extensions in A before (black) and after (cyan) Vc binding. (D) Diagram illustrating the Vc-induced conformational transitions. The extension changes averaged over eight Vc-binding events are indicated.