Table 1.

Percentages of apoptotic cells following a 72-h epirubicin treatment

Cell line	Control	Epirubicin concentration
MDA-MB-231	DMSO	1 nM	25 nM	1000 nM
Native	18 %	17 %	41 %	94 %
25 nM-R	10 %	10 %	8 %	50 %
MCF7	DMSO	1 nM	30 nM	1000 nM
Native	32 %	29 %	49 %	77 %
30 nM-R	20 %	24 %	23 %	78 %
SKBR3	DMSO	1 nM	15 nM	1000 nM
Native	22 %	26 %	24 %	59 %
15 nM-R	18 %	17 %	17 %	34 %
ZR-72-1	DMSO	1 nM	10 nM	1000 nM
Native	36 %	44 %	47 %	71 %
10 nM-R	29 %	28 %	29 %	62 %

DMSO dimethyl sulphoxide; DRFS, distant relapse-free survival

Apoptotic cells = annexin V–positive. Debris and necrotic cells (annexin V–negative, 7-aminoactinomycin D–positive) were gated out. Percentages reported are derived from a single experiment; at least two independent experiments were done for each cell line

Bold data denotes the different concentrations of epirubicin used for each cell line