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. 2016 Feb 5;15:29. doi: 10.1186/s12934-016-0432-8

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© Aw and Polizzi. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Plate and liquid PTVA methodology. Schematic representation of the method for generating multi-copy clones by traditional plate PTVA (left) and liquid PTVA (right) changing medium either every 12 or 24 h. Unless otherwise stated concentrations are for both Zeocin and G418 selection