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. 2016 Feb 5;15:29. doi: 10.1186/s12934-016-0432-8

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© Aw and Polizzi. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Comparison of GFP expression from strains of different copy numbers created using different PTVA methods. Copy number was measured using qPCR and GFP expression by fluorescence. All three methods of PTVA are presented on each graph, liquid 12 h (L12), liquid 24 h (L24) and traditional plate PTVA. a pZGFP-1. b pKGFP-2. c pKαGFP-2