Fig. 4.

Liquid PTVA was successful for pZαGFP when starting with a single copy clone. a Box plots of final copy numbers after traditional plate PTVA and liquid PTVA with medium changes every 24 h (L24) of pZαGFP-1. The box plots indicate the first quartile, median and third quartile, with the whiskers indicating the minimum and maximum (n = 20 for each condition). Red asterisks indicate significant comparisons according to Kruskal-Wallis one-way analysis of variance (p ≤ 0.05) and green asterisks indicate significant comparisons of the median according to Wilcoxon-Mann-Whitney test (p ≤ 0.05). Copy number was determined by qPCR. b GFP expression against copy number measured by fluorescence for both traditional plate PTVA and L24