Fig. 5.

Single copy starting clones result in higher copy number post-PTVA. Box plots of final copy numbers after plate PTVA and 24 h liquid PTVA (L24) for two copy clones versus one copy clones. The box plots indicate the first quartile, median and third quartile, with the whiskers indicating the minimum and maximum (n = 20 for each condition). Copy number was determined by qPCR. Blue indicates copy number of final clones when PTVA was started with a one copy clone strain. Green indicates copy number of clones when PTVA was started with a two copy clone strain. Red asterisks indicate significant comparisons according to Kruskal-Wallis one-way analysis of variance (p ≤ 0.05) and green asterisks indicate significant comparisons of the median according to Wilcoxon-Mann-Whitney test (p ≤ 0.05). a pKGFP. b pKαGFP