Table 2.
Properties of PfCelTOS, human IL-2 and designed constructs determined by ProtParam
| Protein | PfCelTOS | Fusion protein1 | Fusion protein2 |
|---|---|---|---|
| (IL-2-linker-PfCelTOS) | (Co1-linker-IL-2-linker-PfCelTOS) | ||
| No. of amino acids | 173 | 321 | 348 |
| Molecular weight | 19087.4 | 35433.2 | 38176.4 |
| Theoretical pI | 4.58 | 5.11 | 5.32 |
| Total No. of negatively charged residues (Asp + Glu) | 23 | 38 | 41 |
| Total No. of positively charged residues (Arg + Lys) | 13 | 28 | 32 |
| Formula | C855H1326N212O274S4 | C1581H2495N405O494S11 | C1703H2691N441O530S11 |
| Instability index | 41.67 | 48.01 | 49.75 |
| Aliphatic index | 89.48 | 91.06 | 89.11 |
| Grand average of hydropathicity (GRAVY) | −0.106 | −0.151 | −0.172 |