Fig. 5.

Effects of intrathecal injection of miR-320a mimics and AMO on neurological motor function, spinal water content and Evans blue (EB) extravasation after IR. a Effects of intrathecal injection of miR-320a mimics and AMO on neurological motor function after IR. Neurological function scores were assessed at 6 h intervals during the 48 h observation using Tarlov scores after injury in each group. Neurological function scores ranged from 0 (paraplegia) to 4 (normal). Intrathecal infusion of miR-320a mimic markedly improved lower limb motor function, whereas injection of AMO-320a reversed effects. **P < 0.05 versus Sham group; ^## P < 0.05 versus IR group. b Quantification of water content (%) of the spinal cord. c Effects of intrathecal injection of miR-320a mimics and AMO on EB extravasation after IR. Scale bar 50 μm. d The EB content of the spinal cord (μg/g). e Quantification of EB fluorescence density (INT/mm²). Compared with IR group, EB extravasation (red) and water content were significantly lower in rats intrathecal pretreatment with mimic-320a, and conversely higher in rats intrathecally receiving AMO-320a at both above time. **P < 0.05 versus Sham group; ^## P < 0.05 versus IR group