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. 2016 Feb 6;15:30. doi: 10.1186/s12934-016-0431-9

Table 2.

Differential expression of genes involved in fatty acid metabolism in C. psychrerythraea without or with 12 μg/mL cerulenin at 10 °C

Locus ID Gene ID Gene description Fold change (log2)
Gene involved in fatty acid biosynthesis
 CPS_RS06985 accA acetyl-CoA carboxylase subunit A 0.28
 CPS_RS04205 accB acetyl-CoA carboxylase subunit B 0.47
 CPS_RS04200 accC acetyl-CoA carboxylase subunit C 0.39
 CPS_RS17005 accD acetyl-CoA carboxylase subunit D −0.31
 CPS_RS14645 fabA 3-hydroxydecanoyl-ACP dehydratase, isomerase −0.21
 CPS_RS17030 fabB 3-ketoacyl-ACP synthase I 0.96
 CPS_RS10240 fabF 3-ketoacyl-ACP synthase II 0.41
 CPS_RS09575 fabH 3-ketoacyl-ACP synthase III 0.38
 CPS_RS10225 fabD malonyl-CoA:ACPtransacylase 0.95
 CPS_RS07145 fabG1 3-ketoacyl-ACP reductase 0.01
 CPS_RS10230 fabG2 NADPH-dependent 3-ketoacyl-ACPreductase −0.03
 CPS_RS06960 fabZ 3-hydroxyacyl-ACP dehydratase −0.06
 CPS_RS01535 fabR DNA-binding transcriptional repressor −0.04
Gene involved in fatty acid degradation
 CPS_RS01760 fadA 3-ketoacyl-CoA thiolase −0.57
 CPS_RS01765 fadB 3-hydroxyacyl-CoA dehydrogenase −0.92
 CPS_RS05300 fadD1 Long chain fatty acid-CoA ligase −1.51
 CPS_RS15290 fadD2 Long chain fatty acid-CoA ligase 0.28
 CPS_RS10085 fadE Acyl-CoA dehydrogenase 0.40
 CPS_RS04110 fadH 2,4-dienoyl-CoA reductase 0.55
 CPS_RS15575 fadR Fatty acid metabolism regulator −0.39
Gene involved in fatty acid modification or lipid metabolism
 CPS_RS00390 cti fatty acid cis/trans isomerase 0.11
 CPS_RS10220 plsX glycerol-3-phosphate acyltransferase −0.30
 CPS_RS19415 plsY glycerol-3-phosphate acyltransferase 0.31
 CPS_RS00605 GPAT glycerol-3-phosphate acyltransferase 0.21
 CPS_RS04525 LPAAT 1-acyl-sn-glycerol-3-phosphate acyltransferase 0.06
 CPS_RS21310 LPAAT 1-acyl-sn-glycerol-3-phosphate acyltransferase −0.08
 CPS_RS04435 acyltransferase 0.02
 CPS_RS08065 acyltransferase 0.25
 CPS_RS14560 acyltransferase 0.55
 CPS_RS21745 acyltransferase 0.52
 CPS_RS22505 acyltransferase 0.70
 CPS_RS22520 acyltransferase −0.20
Genes involved in PKS pathway
 CPS_RS13885 pfaA polyunsaturated fatty acid synthase −0.58
 CPS_RS13880 pfaB polyunsaturated fatty acid synthase −0.37
 CPS_RS13875 pfaC polyunsaturated fatty acid synthase 0.03
 CPS_RS13865 pfaD polyunsaturated fatty acid synthase 0.60
 CPS_RS13895 pfaE 4′-phosphopantetheinyl transferase −0.51

Fold change (log2 values) in transcript levels under specified conditions as determined by RNA-seq. Transcript abundance obtained from RNA-seq data is indicated as RPKMs (See “Methods” section)