Table 2.
Differential expression of genes involved in fatty acid metabolism in C. psychrerythraea without or with 12 μg/mL cerulenin at 10 °C
| Locus ID | Gene ID | Gene description | Fold change (log2) |
|---|---|---|---|
| Gene involved in fatty acid biosynthesis | |||
| CPS_RS06985 | accA | acetyl-CoA carboxylase subunit A | 0.28 |
| CPS_RS04205 | accB | acetyl-CoA carboxylase subunit B | 0.47 |
| CPS_RS04200 | accC | acetyl-CoA carboxylase subunit C | 0.39 |
| CPS_RS17005 | accD | acetyl-CoA carboxylase subunit D | −0.31 |
| CPS_RS14645 | fabA | 3-hydroxydecanoyl-ACP dehydratase, isomerase | −0.21 |
| CPS_RS17030 | fabB | 3-ketoacyl-ACP synthase I | 0.96 |
| CPS_RS10240 | fabF | 3-ketoacyl-ACP synthase II | 0.41 |
| CPS_RS09575 | fabH | 3-ketoacyl-ACP synthase III | 0.38 |
| CPS_RS10225 | fabD | malonyl-CoA:ACPtransacylase | 0.95 |
| CPS_RS07145 | fabG1 | 3-ketoacyl-ACP reductase | 0.01 |
| CPS_RS10230 | fabG2 | NADPH-dependent 3-ketoacyl-ACPreductase | −0.03 |
| CPS_RS06960 | fabZ | 3-hydroxyacyl-ACP dehydratase | −0.06 |
| CPS_RS01535 | fabR | DNA-binding transcriptional repressor | −0.04 |
| Gene involved in fatty acid degradation | |||
| CPS_RS01760 | fadA | 3-ketoacyl-CoA thiolase | −0.57 |
| CPS_RS01765 | fadB | 3-hydroxyacyl-CoA dehydrogenase | −0.92 |
| CPS_RS05300 | fadD1 | Long chain fatty acid-CoA ligase | −1.51 |
| CPS_RS15290 | fadD2 | Long chain fatty acid-CoA ligase | 0.28 |
| CPS_RS10085 | fadE | Acyl-CoA dehydrogenase | 0.40 |
| CPS_RS04110 | fadH | 2,4-dienoyl-CoA reductase | 0.55 |
| CPS_RS15575 | fadR | Fatty acid metabolism regulator | −0.39 |
| Gene involved in fatty acid modification or lipid metabolism | |||
| CPS_RS00390 | cti | fatty acid cis/trans isomerase | 0.11 |
| CPS_RS10220 | plsX | glycerol-3-phosphate acyltransferase | −0.30 |
| CPS_RS19415 | plsY | glycerol-3-phosphate acyltransferase | 0.31 |
| CPS_RS00605 | GPAT | glycerol-3-phosphate acyltransferase | 0.21 |
| CPS_RS04525 | LPAAT | 1-acyl-sn-glycerol-3-phosphate acyltransferase | 0.06 |
| CPS_RS21310 | LPAAT | 1-acyl-sn-glycerol-3-phosphate acyltransferase | −0.08 |
| CPS_RS04435 | acyltransferase | 0.02 | |
| CPS_RS08065 | acyltransferase | 0.25 | |
| CPS_RS14560 | acyltransferase | 0.55 | |
| CPS_RS21745 | acyltransferase | 0.52 | |
| CPS_RS22505 | acyltransferase | 0.70 | |
| CPS_RS22520 | acyltransferase | −0.20 | |
| Genes involved in PKS pathway | |||
| CPS_RS13885 | pfaA | polyunsaturated fatty acid synthase | −0.58 |
| CPS_RS13880 | pfaB | polyunsaturated fatty acid synthase | −0.37 |
| CPS_RS13875 | pfaC | polyunsaturated fatty acid synthase | 0.03 |
| CPS_RS13865 | pfaD | polyunsaturated fatty acid synthase | 0.60 |
| CPS_RS13895 | pfaE | 4′-phosphopantetheinyl transferase | −0.51 |
Fold change (log2 values) in transcript levels under specified conditions as determined by RNA-seq. Transcript abundance obtained from RNA-seq data is indicated as RPKMs (See “Methods” section)