Parental, ΔUL56, ΔUS9, or a ΔUL56/ΔUS9
double-mutant virus was injected into the anterior chamber of the eye of male
Long Evans rats. The location of the injection in the eye is indicated by an
asterisk (*). Animals were sacrificed at the indicated times and the
superior cervical ganglion (SCG) was removed, sectioned, and scored for infected
neurons based on red-fluorescence emissions from the pUL25/mCherry capsid
fusion. The symbol ‘†’ denotes the time of imminent
death from infection. The lower dashed line indicates the number of neurons in
the SCG that project directly to the eye. The upper dashed line indicates the
total number of neurons, ≅ 30,000, that comprise the SCG.