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. Author manuscript; available in PMC: 2017 Feb 15.
Published in final edited form as: J Immunol. 2016 Jan 15;196(4):1891–1899. doi: 10.4049/jimmunol.1501555

Figure 2.

Figure 2

Characterization of a novel transgenic mouse model of lung epithelial p52 expression. A) Western blot demonstrating FLAG-tagged p52 transgene expression in lung nuclear protein from WT and CCSP-p52 mice on dox for 1 week. B) FLAG immunostaining of lung sections from WT and CCSP-p52 mice on dox for 1 week (20x magnification). C) Western blots for NF-κB family members using lung nuclear protein from mice on dox for 1 week. Three separate Western blots were performed, and TATA Binding Protein (TBP) was probed as a loading control for nuclear protein on each membrane. D) Total BAL inflammatory cell numbers and E) representative lung photomicrographs of H&E-stained lung sections (40x magnification) from WT and CCSP-p52 mice (for no dox and 1 month n=3–7/group, for 1 week n=10–13/group).