TABLE 1.
Primers used in this study.
| Name | Position | Sequence | Assay |
|---|---|---|---|
| oVM12 | 82699-82718 | 5′-CTCAGACTCCCTGCGAGCAT-3′ | 3′RACE |
| oVM36 | 83438-83454 | 5′-TGGAACATCACAGCTTG-3′ | 3′RACE, RPAa |
| oVM67 | 83254-83274 | 5′-GCATGTAACCTTCTTGGCGAG-3′ | 3′RACE |
| oVM90 | 83705-83687 | 5′-taatacgactcactatag/GGCGGTCTGGTGTTGTGTT-3′ | RPAa |
| oVM96 | 82675-82695 | 5′-tactcagaattcaccatg/GGTGTGTCTGACGCCGTAAAG-3′ | RT-PCR, 3′RACE |
| oNP18 | 83606-83583 | 5′-atcgtgttaac/GAGAAAGGCTACGTGAGTTACATG-3′ | RT-PCR |
| oVM280 | 83667-83647 | 5′-GAGAAGAGACCACGCCTGACT-3′ | RT-PCRb |
| oZMZ296 | N/A | 5′-gcatggcggacttgaagaa-3′ (vector) | RT-PCR |
| oVM79 | N/A | 5′-gggcactggagtggcaac-3′ (vector) | RT-PCR |
| BGH | N/A | 5′-tagaaggcacagtcgagg-3′ (vector) | RT-PCR |
Primer positions are based on KSHV genome sequence GenBank Acc. No. U75698.1. The lower case marks non-KSHV sequences.
a
primers used to prepare DNA template for RNA probe by in vitro transcription, with oVM90 attached by a T7 promoter sequence;
b
primer identical to Rev-C from (Arias, Weisburd et al., 2014). BGH, bovine growth hormone sequence from the vector.