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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Anal Biochem. 2015 Dec 23;496:43–49. doi: 10.1016/j.ab.2015.11.027

Fig. 5.

Fig. 5

Glycosyltransferase activity of PNPase. An assay mixture containing 100 mM Tris (pH 8), 0.15 mM thymine, 0.25 mM inosine, and 500 mU/mL of PNPase was prepared, added to sample and reference cuvettes to record a baseline in the 250–450 nm wavelength interval. 500 mU/mL of XOD were then added to the sample cuvette, and difference spectra were recorded every 30 min after enzyme addition.