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. Author manuscript; available in PMC: 2017 Jan 15.
Published in final edited form as: Virology. 2015 Nov 21;488:61–67. doi: 10.1016/j.virol.2015.10.033

Figure 2. Development of a sensitive PCR assay to detect latent HSV-2 DNA.

Figure 2

A) A representative qPCR standard curve generated using NaI gradient double-banded HSV-2 DNA and ICP8 primers and probes showing 6 logs of detection with a lower limit of 1 copy/reaction and an efficiency of 96%. B) An independent experiment showing detection of latent HSV-2 DNA in mouse DRGs infected with HSV-2 186ΔKpn. Green circles correspond to standard points ranging from 2 to 2×106 copies. Black crosses correspond to values for experimental samples determined to contain from 3.8 to 12 copies per reaction.