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. 2015 Sep 4;103(11):3649–3658. doi: 10.1002/jbm.a.35505

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© 2015 The Authors Journal of Biomedical Materials Research Part A Published by Wiley Periodicals, Inc.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Bone formation in critical‐size rat calvarial defects. A: Three‐dimensionally reconstructed high‐resolution μCT image of defects implanted with cells/scaffolds after 8 weeks of healing. Note the new bone formed in the four groups; with osteogenic medium (+OM) or without osteogenic medium (−OM) with different densities of cell seeding (low density (LD) or high density (HD)). B: Quantification of percentage of area and volume of bone regeneration in calvarial defects after 8 weeks of healing. Implantation of scaffolds containing cells seeded at high density and cultured with osteogenic medium exhibit a significant percentage of bone volume (p = 0.038). [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]