Figure 5.

Representative sections of Masson's trichrome staining through calvarial defects at 8 weeks (×10). A: Section of a defect implanted with a scaffold containing cells cultured without osteogenic medium and seeded at low density, showing fibrous connective tissue and collagen. B: Defect implanted with a scaffold containing cells cultured without osteogenic medium and seeded at high density, showing osteoid‐like tissue (green areas). C: Defect implanted with a scaffold containing cells cultured with osteogenic medium and seeded at low density, showing osteoid‐like tissue. D: Defect implanted with a scaffold containing cells cultured with osteogenic medium and seeded at high density, showing formation of a bridge of bone‐like tissue. Scale bar = 1000 μm. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]