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. 2015 Jun 25;36(18):2269–2285. doi: 10.1002/elps.201500011

Figure 2.

Figure 2

Profiles of BM analyzed via: (A) Median 1H NMR spectrum of aqueous fraction of BM, 1 = Fucose, 2 = Lactate, 3 = Alanine, 4 = Butyrate, 5 = N‐acetyl glutamine, 6 = Acetate, 7 = N‐acetyl neuraminic acid, 8 = Glutamine, 9 = Glutamate, 10 = Citrate, 11 = Creatine, 12 = Creatinine, 13 = Choline, 14 = Phosphocholine, 15 = Glycerophosphocholine, 16 = Lactose, 17 = Taurine, 18 = Glucose, 19 = Galactose. (B) Median 1H NMR spectrum of lipid fraction of BM 1 = Cholesterol, 2 = Terminal CH3, 3 = Omega 3 terminal CH3, 4 = Saturated CH2, 5 = CH 2‐CH2‐COOH, 6 = Unsaturated fatty acids CH 2‐CH = CH, 7 = CH 2‐COOH, 8 = Docosahexaenoic acid, = CH‐CH 2‐CH 2‐COOH, 9 = Polyunsaturated fatty acid, = CH‐CH2‐CH = , 10 = Phosphatidylcholine, ‐N(CH 3)3, 11 = Phospholipid, 12 = Glyceryl C1,3, 13 = Glyceryl C2, 14 = Unsaturated fatty acids, ‐CH = CH‐. (C) Aqueous fraction of BM analysed using CE‐MS in positive ionization mode; 1 = Creatinine, 2 = Lysine, 3 = Nicotinamide, 4 = Arginine, 5 = Carnitine, 6 = Acetyl‐L Carnitine, 7 = Cytosine, 8 = Cytidine, 9 = Creatine, 10 = Alanine, 11 = 5‐amino Valeric acid/valine, 12 = N‐methyl‐L‐Valine/leucine/isoleucine, 13 = Serine, 14 = Tryptophan, 15 = Threonine, 16 = Glycyl‐glycine, 17 = Methionine, 18 = Citrulline, 19 = Phenylalanine, 20 = Glutamic acid, 21 = Tyrosine, 22 = Cystine, 23 = Aspartic acid. (D) UPLC‐MS; lipid fraction of BM analysed in negative ionization mode, tentative identifications are shown in Supporting Information Table 4. (E) UPLC‐MS; lipid fraction analyzed using positive mode, tentative identifications are shown in Supporting Information Table 4. (F) GC‐MS single phase assignments from 6–19 min 1 = Pyruvic acid, 2 = Lactic acid (Standard confirmed), 3 = Glycolic acid, 4 = Valine 1, 5 = Alanine 1, 6 = 2‐Hydroxybutyric acid, 7 = 2‐Furoic acid, 8 = Isoleucine 1, 9 = Valine 2, 10 = Urea (Standard confirmed), 11 = Benzoic acid, 12 = Caprylic acid, 13 = Glycerol (Standard confirmed), 14 = Phosphoric acid, 15 = Proline 2 (Standard confirmed), 16 = Glycine, 17 = Succinic acid, 18 = Glyceric acid, 19 = Serine 2 (Standard confirmed), 20 = Threonine 2, 21 = Capric acid, 22 = Malic acid, 23 = Adipic acid, 24 = Threitol, 25 = Pyroglutamic acid, 26 = Glutamic acid 1, 27 = Creatinine, 28 = Glutamic acid 2 (Standard confirmed), 29 = Lauric acid, 30 = Lyxose 1/Lyxosylamine 1, 31 = Lyxose 2/Lyxosylamine 2/Ribose, 32 = Xylitol, 33 = Fucose 1, 34 = Fucose 2, 35 = Citric acid (Standard confirmed), 36 = Hippuric acid 2, 37 = Myristic acid, 38 = Tagatose 1/Sorbose 2/Sorbose 1/Fructose 1, 39 = Tagatose 2/Fructose 2/Fructose 1, 40 = Galactose 1/ Mannose 1/Allose 1/Gluconic acid lactone 1, 41 = Glucose 1/Talose 1 (Standard confirmed), 42 = Altrose 2/Mannose 2/Glucose 2/Allose 2/Talose 2, 43 = Mannitol/Sorbitol, 44 = 1‐Hexadecanol, 45 = Palmitoleic acid, 46 = Palmitic acid.