Figure 1.

Tissue Distribution of ILC Subsets. Average percent frequencies of NK, ILC1, ILC2, and ILC3 in total ILCs in indicated tissues of C57BL/6 mice are shown (n ≥ 4; 7–10 weeks of age). Total ILC frequencies among CD45⁺ leukocytes are also shown. NK cells were identified based on CD3⁻CD19⁻CD127⁻NKp46⁺NK1.1⁺ (most tissues), CD3⁻CD19⁻RORγt⁻NKp46⁺NK1.1⁺ (intestinal LP), or CD3⁻CD19⁻CD127⁻RORγt⁻NKp46⁺NK1.1⁺ Eomes⁺ (IE) phenotype as described before [17,36]. ILC1 were identified by Lin⁻RORγt⁻CD127⁺NKp46⁺NK1.1⁺ (most tissues) or Lin⁻RORγt⁻CD127⁻NKp46⁺NK1.1⁺ (IE) phenotype as described before [17,18,36]. ILC2 were identified by Lin⁻CD127⁺CD90⁺KLRG1⁺GATA3⁺ (most tissues), Lin⁻CD90⁺CD25⁺GATA3⁺ (spleen and skin), or Lin⁻CD127⁺CD90⁺T1/ST2⁺GATA3⁺ (lungs and WAT) phenotype [10,20,37,47,93]. ILC3 were identified by Lin⁻GATA3⁻CD127⁺CD90⁺RORγt⁺ phenotype [20]. ILC3 subsets within the total ILC3 group were not examined separately. The ILC1 population in the bone marrow includes ILC1 progenitors. Abbreviations: BM, bone marrow; IE, intraepithelial compartment; ILC, innate lymphoid cell; LP, lamina propria; MLN, mesenteric lymph node; NK, natural killer cell; PLN, peripheral lymph node (inguinal LN).