Figure 3.

Mesna downregulates neutrophils and upregulates iNOS in the DSS‐induced colonic ulcers. (a) Immunohistochemically labelled MPO+ cells (neutrophils) at the pyogranulomatous tissue of colonic mucosa ulcer beds. Treatment schemes containing Mesna decrease neutrophils at statistically significant levels. (b) iNOs‐specific immunohistochemistry of DSS‐induced ulcers. Mesna administration correlates with a significant increase of i‐NOS positive cells. IHC, diaminobenzidine chromogen, haematoxylin counterstain. Scale bars: 25 μm. Numbers in the bottom of figures and the y‐axis of bar graphs depict the mean ± SEM of IHC‐labelled cells in high‐power magnification images. The asterisks indicate statistical significance between each experimental group with the control colitic rat group (black bar); **P < 0.01, ***P < 0.001.