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. 2016 Jan 19;96(6):427–432. doi: 10.1111/iep.12162

Figure 1.

Figure 1

(a) miR‐1 expression in SUNE‐1, 5‐8F, HONE‐1, C666‐1 and nasopharyngeal epithelial (NPE) cell line NP‐69. (b) Western blot assay showed that C666‐1 cells expressed higher levels of LMP1, when compared with other NPC cell lines and NP‐69. (c) NP‐69 cells were transiently transfected with pcDNA3.1‐LMP1 or empty vector pcDNA3.1, and the overexpression efficiency of LMP1 was confirmed by Western blot analysis. (d) miR‐1 expression in NP‐69‐pcDNA3.1‐LMP1 cells was decreased at both the primary and mature transcript levels, when compared with that in NP‐69‐pcDNA3.1 cells. (e) siRNA was used to suppress the expression of Stat‐3 in C666‐1 cells. (f) miR‐1 expression was markedly increased when Stat‐3 was knocked down in C666‐1 cells. * and ** represent significance.