FIGURE 2.

Purification and identification of active component of EPS273. (A) The profiles of the fractions in the gel filtration, which were collected and monitored for the biofilm formation determined at OD595 nm after crystal violet staining and polysaccharide content determined at OD490 nm after the phenol-sulfuric acid assay. (B) Effects of Proteinase K, DNase I, RNase A and NaIO₄ on the activities of EPS273 inhibiting biofilm formation of P. aeruginosa PAO1. EPS273 (0.1 μg/mL) was, respectively, treated with proteinase K (PrK) (1 mg/mL), DNaseI (100 μg/mL), RNaseA (100 μg/mL) for 1 h or NaIO₄ (10 mM) for 12 h at 37°C, then taken to measure the antibiofilm activity. Error bars represent standard deviations of three independent experiments. Error bars indicate the standard deviations of three measurements. ^∗P < 0.05 versus the control.