FIG. 5.

Quantification of axon thickness on micropatterned PDMS substrate. (a) Phase-contrast images (10×) of micropatterned channels of 50 μm (left) and 200 μm (right) widths; (b) DRG axons on channels of 50 μm (left) and 200 μm (right) widths after 21 days of culture were stained with anti-mouse β-III tubulin and imaged with confocal microscopy (20×); and (c) comparison of axon thickness on the channels versus on the prestretched PDMS substrate after 21 days of culture. ANOVA-Tukey's test was applied to assess for significant difference. **p < 0.01. Color images available online at www.liebertpub.com/tec