Analysis of GIPR expression in areas neighboring the site of injury. Representative image of GIPR immunostaining in spinal cord of sham-operated (A) or SCI animals 2 h (B), 6 h (C), 24 h (D), 14 days (E), and 28 days (F) after surgery. (G) Semi-quantitative analysis of GIPR levels in areas neighboring the site of injury. Data are shown as mean ± SEM, n = 5 animals/group. Results are represented as arbitrary units of optical density. *P ≤ 0.05 in Student's t test comparing SCI with the corresponding sham-operated groups. GIPR, glucose-dependent insulinotropic peptide receptor; SCI, spinal cord injury; SEM, standard error of mean. (Original magnification 400×.)