FIG. 5.

Analysis of GIP expression in areas neighboring the site of injury. Representative images of GIP immunoreactivity in the spinal cord of sham-operated (A) or SCI animals assessed 2 h (B), 6 h (C), 24 h (D), 14 days (E), or 28 days (F) after surgery. (G) Semi-quantitative analysis of GIP levels in areas neighboring the site of injury. Each column represents the group mean and vertical lines represent the SEM of data obtained from five animals. Results are represented as arbitrary units of optical density. *P ≤ 0.05 in Student's t test comparing SCI with the corresponding sham-operated groups. GIP, glucose-dependent insulinotropic peptide; SCI, spinal cord injury; SEM, standard error of mean. (Original magnification 400×.)