FIG. 6.

Analysis of GIP expression in the injury epicenter. Representative images of GIP immunostaining in the spinal cord of sham-operated (A) or SCI animals assessed 2 h (B), 6 h (C), 24 h (D), 14 days (E), or 28 days (F) after surgery. The injury epicenter of SCI animals show granular staining in the cytoplasm and in extensions of neuronal cells. (G) Semi-quantitative assessment of GIP in the neuronal extensions present in the injury epicenter or in the gray matter of sham-operated animals. Data are shown as mean ± SEM, n = 5 animals/group. Results are represented as arbitrary units of optical density. *P ≤ 0.05 in Student's t test comparing SCI with corresponding sham-operated groups. GIP, glucose-dependent insulinotropic peptide; SCI, spinal cord injury; SEM, standard error of mean. (Original magnification 400×.)