FIG. 7.

Detection of neural progenitors in the medullary canal of the injured spinal cord. Representative images of nestin immunostaining in ependymal cells of the spinal cord canal of sham-operated (A) or SCI animals assessed 2 h (B), 6 h (C), 24 h (D), 14 days (E), or 28 days (F) after surgery. (G) Semi-quantitative analysis of nestin immunostaining. Data are shown as mean ± SEM, n = 5 animals/group. Results are represented as arbitrary units of optical density. *P ≤ 0.05 in Student's t test comparing SCI with corresponding sham-operated groups. With arrows highlight positive-stained cells. SCI, spinal cord injury; SEM, standard error of mean. (Original magnification 400×.)