FIG 1.
Fiber modification of the Ad5 vector vaccine enhances the expression of FMDV structural proteins in mammalian cells. (A and B) SDS-PAGE analysis of VP1 and VP3 expression. CHO and CHO-αVβ6 cells (A) and LF-PK and LF-PK-αVβ6 cells (B) were infected with either AdtO1C.2B or AdtO1C.2B.RGD at the indicated MOIs. Twenty-four hours postinfection, cell lysates were prepared and proteins were resolved by SDS-PAGE and visualized by Western blotting using polyclonal antibodies against FMDV capsid proteins VP1 and VP3. (C) Quantitation of FMDV VP1 protein in panels A and B by densitometry. (D) Relative quantitation of Ad5 hexon protein detected in infected (MOIs of 500) LF-PK, LF-PK-αVβ6, CHO, and CHO-αVβ6 cells. All experiments depicted were repeated at least three times, with similar results. Error bars represent standard deviation of the mean. Statistical significance was determined with Student's t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001.