TABLE 1.
Primers used for cytokine profiling
| Gene | Primer and probe set | Sequence | GenBank accession no. |
|---|---|---|---|
| CCL3 | Bos taurus CCL3 F | AGCCAGGTCTTCTCGGCAC | EU276059 |
| Bos taurus CCL3 R | AGAAGCAGCAGGCCGTTG | ||
| Bos taurus CCL3 T | ATTTGGCGCTGACACC | ||
| CCL5 | Bos taurus CCL5 F | CGCTCCTGCTTCTGCCTC | BC102064 |
| Bos taurus CCL5 R | TATAGGCAAAGCAGCAGGGC | ||
| Bos taurus CCL5 T | CATATGCCTCGGACACC | ||
| IL-1B | Bos taurus IL-1B F | AACTGGTACATCAGCACTTCTCAAA | EU276067.1 |
| Bos taurus IL-1B R | ATATCCTGGCCACCTCGAAA | ||
| Bos taurus IL-1B T | AGAAAGGCCCGTCTTCCTGGGACA | ||
| IL-6 | Bos taurus IL-6 F | GACACTGGCAGAAAATAAGCTGAA | NM_173923.2 |
| Bos taurus IL-6 R | TCGCCTGATTGAACCCAGA | ||
| Bos taurus IL-6 T | CAAAAATGGAGGAAAAGGACGGATGCTT | ||
| CXCL10 | Bos taurus RIG-I F | GTCATTCCTGCAAGTCAATCCTG | EU276062 |
| Bos taurus RIG-I R | CCCATTCTTTTTCATTGTGGC | ||
| Bos taurus RIG-I T | CCACGTGTCGAGATTA | ||
| IRF-1 | Bos taurus IRF-1 F | CCGGTGGAAATTGTGCCA | BC102766 |
| Bos taurus IRF-1 R | GGGCGACACCTGAAAGTTGT | ||
| Bos taurus IRF-1 T | CAGCACCAGCGACC | ||
| IRF-7 | Bos taurus IRF-7 F | GGACTGTGACACGCCCATCT | BC151518 |
| Bos taurus IRF-7 R | CCCGGAACTCCAGCAGTTC | ||
| Bos taurus IRF-7 T | ACTTCGGCACCTTCT | ||
| TNF | Bos taurus TNF F | AGCCTCAAGTAACAAGCCGGT | EU276079 |
| Bos taurus TNF R | TGCCCCGGAGAGTTGATG | ||
| Bos taurus TNF T | CCCACGTTGTAGCCG | ||
| IFN-γ | Bos taurus IFN-γ F | GGAGGACTTCAAAAAGCTGATTCA | FJ263670 |
| Bos taurus IFN-γ R | GCTTTGCGCTGGATCTGC | ||
| Bos taurus IFN-γ T | TTCCGGTGGATGATC | ||
| IL-10 | Bos taurus IL-10 F | GGTGATGCCACAGGCTGAG | EU276074 |
| Bos taurus IL-10 R | AGCTTCTCCCCCAGTGAGTTC | ||
| Bos taurus IL-10 T | CCACAGGCTGAGAACCACGGGC | ||
| IFN-α | Bos taurus IFN-α F | AGCACACCTTCCAGCTTTTCA | XM_001250455 |
| Bos taurus IFN-α R | GGAGGCTCTTGTCCCACACA | ||
| Bos taurus IFN-α T | CAGAGGGCTCGGCC | ||
| IFN-β | Bos taurus IFN-β F | CTACAGCTTGCTTCGATTCCAA | M15477 |
| Bos taurus IFN-β R | CTGCCCCAGGAGTTTCTGAC | ||
| Bos taurus IFN-β T | ACGTCAGAGCCTTAAA | ||
| CD80 | Bos taurus CD80 F | GGCTCTCTCAGCTCTTGGTG | NM_001206439 |
| Bos taurus CD80 R | TTTGCCAATAGATCCGAAGG | ||
| Bos taurus CD80 T | ACCCCAAAGAGTGTGACCAA | ||
| CD86 | Bos taurus CD86 F | CCAAACACCCAAAACCTCAG | NM_001038017 |
| Bos taurus CD86 R | CAAGGTCCAACTGTCCTGGT | ||
| Bos taurus CD86 T | TCCCAAGTATATAGGCCGCA | ||
| CCR7 | Bos taurus CCR7 F | CAAGAAGGATGTGCGGAACT | NM_001024930 |
| Bos taurus CCR7 R | GGGAGGGTCAGAAGGAAGAG | ||
| Bos taurus CCR7 T | CGTACCTGCTCAACCTAGCC |