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. 2016 Feb 8;6:20577. doi: 10.1038/srep20577

Table 1. Production of recombinant VZV membrane proteins and mAbs.

Protein name Expression system Expression segment (aa) Fusion tag Expression form Purify method mAbs
rgE Baculovirus 1–537 His tag Secretion expression Ni-NTA 70 41
rgB E. coli 23–382 His tag Inclusion body Electroelution 5
rgH E. coli 518–804 His tag Inclusion body Ni-NTA 3
rgI E. coli 21–274 GST tag Soluble expression Glutathione Sepharose 4B 2
rgC E. coli 18–407 GST tag Soluble expression Glutathione Sepharose 4B 3
rgK E. coli 22–114 GST tag Inclusion body Electroelution 2
rgL E. coli 23–160 His tag Inclusion body Ni-NTA 4
rgM E. coli 2–35 GST tag Soluble expression Glutathione Sepharose 4B 3
rgN E. coli 21–49 His tag Soluble expression Ni-NTA 2
VZV gps         Lentil lectin Sepharose 4B 7
Cell-free virus           9