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. 2016 Feb 8;6:20505. doi: 10.1038/srep20505

Figure 4. EP2-deficiency impairs IgE production mainly by affecting IgE class switching.

Figure 4

Splenic B cells from EP2 knockout (KO) mice or wild type (WT) littermates were stimulated with LPS or LPS+IL-4 in the presence or absence of PGE2. (A,B) RNA was prepared from day 3 and day 5 cultures and examined for germline transcripts ε (GLTε) (A) and post switch transcripts ε (PSTε) (B), respectively. The expression levels were calibrated against that in LPS+IL-4-treated wild type B cells. Data from at least four independent experiments (each with duplicates) are presented as mean ± SEM. (C,D) Cell proliferation was measured by CSFE dilution. Representative histograms (C) and the percentage of cells undergoing at least one division (D) are shown. (E,F) Cell apoptosis was analyzed by Annexin-V and 7-AAD staining. Representative dot plots (E) and the percentage of Annexin-V+ cells (F) are shown. Experiments were repeated at least three times. Data are presented as mean ± SEM. *P < 0.05. **P < 0.01. ***P < 0.005. ns, not significant.