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. 2016 Feb 8;6:20505. doi: 10.1038/srep20505

Figure 5. EP2-mediated signal enhances STAT6 phosphorylation and AID expression but inhibits CD23 expression.

Figure 5

Splenic B cells from EP2 knockout (KO) mice or wild type (WT) littermates were stimulated with LPS or LPS+IL-4 with or without PGE2. (A) Cells were harvested at 0, 20, 40, 60 and 80 min after stimulation. Phosphorylated and total STAT6 was detected by Western blotting. The cropped gels have been run under the same experimental conditions. Representative blots are shown of three independent experiments. The numbers indicate the ratio of phosphorylated versus total STAT6 under various conditions. (B,C) RNA was prepared from day 3 cultures and examined for STAT6 (C) and AID mRNA expression by quantitative PCR. The expression levels were calibrated against that in LPS+IL-4-treated wild type B cells. (D,E) Surface expression of CD23 was determined by flow cytometry at day 3. Representative histogram (D) and the percentage of CD23+ cells (E) are shown. Data from at least four independent experiments are presented as mean ± SEM. *P < 0.05. **P < 0.01. ***P < 0.005. ns, not significant.