Figure 1.

Donor BD significantly induces inflammatory candidate markers in the pancreas mRNA expression levels of IL‐1ß, IL‐6, VCAM‐1, and P‐selectin in pancreatic grafts. Significant changes in IL‐1ß and IL‐6 were observed for BD only grafts in comparison to sham and naïve animals (p < 0.001 and p < 0.01, respectively). Although both adhesion molecules VCAM‐1 and P‐selectin were clearly induced, statistical significance was reached only for BD only versus naïve animals (p < 0.01 and p < 0.05, respectively). No significant changes in gene expression were detected in pancreatic grafts derived from the transplanted group. Data are presented as mean values of n = 6–8 animals/group ± SEM. Statistically significant differences were tested with one‐way ANOVA and the Tukey posttest; *p < 0.05, **p < 0.01, ***p < 0.001. ANOVA, analysis of variance; BD, brain dead; BH4, tetrahydrobiopterin; HPRT, hypoxanthine hosphoribosyltransferase; Tx, transplantation.