Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jul 3;78(3):426–438. doi: 10.1002/ana.24453

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 The Authors Annals of Neurology published by Wiley Periodicals, Inc. on behalf of American Neurological Association.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

PMC Copyright notice

Neural‐specific ablation of C9orf72 does not cause signs of neuroinflammation or other pathological hallmarks of amyotrophic lateral sclerosis in 18‐month‐old Nestin‐Cre^+/−;C9orf72^fl/fl mice. (A–D) Immunofluorescent staining of adult spinal cord using anti–glial fibrillary acidic protein (GFAP) or anti‐Iba1 antibodies. Graphs show quantification of GFAP‐ or Iba1‐positive areas. No signs of gliosis are observed in Nestin‐Cre^+/−;C9orf72^fl/fl mice. (E, F) Immunofluorescent staining of adult spinal cord using anti–TDP‐43 antibodies. Nuclear TDP‐43 staining is detected in Nestin‐Cre^+/−;C9orf72^fl/fl mice and control littermates. (G, H) Representative images showing immunostaining for ubiquitin in adult spinal cord. Ubiquitin expression is not affected by loss of C9orf72. Data in graphs represent mean ± standard deviation, n = 3 per genotype. Scale bars: A, C, G, 100μm; E, 30μm. DAPI = 4′,6‐diamidino‐2‐phenylindole; GM = gray matter; WM = white matter.