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. 2015 Jul 3;78(3):426–438. doi: 10.1002/ana.24453

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© 2015 The Authors Annals of Neurology published by Wiley Periodicals, Inc. on behalf of American Neurological Association.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Immunostaining for glial fibrillary acidic protein (GFAP) in the brain of 18‐month‐old mice. Immunofluorescent staining is shown for GFAP in frontal cortex, motor cortex, and hippocampus of Nestin‐Cre^+/−;C9orf72^+/+ mice (A–C″) and Nestin‐Cre^+/−;C9orf72^fl/fl mice (D–F″). Boxes in A–C and D–F are shown at higher magnification in A′–C″ and D′–F″, respectively. No changes in the distribution, number, or appearance of GFAP‐positive astrocytes were detected in Nestin‐Cre^+/−;C9orf72^fl/fl mice. Scale bars: A, D, 200μm; A′, D′, 100μm. DAPI = 4′,6‐diamidino‐2‐phenylindole.