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. 2015 Nov 6;17(1):21–39. doi: 10.1111/tra.12340

Figure 3.

TRA-12340-FIG-0003-b

Effect of endocytosis inhibitors on uptake of transferrin and glycoproteins into HFF ‐Tert cells. A) Cells were incubated with DMSO, 15 µm Dynole or Dynole negative control and 30 µm PitStop2 or its negative control for 15 min. Next transferrin conjugated to Alexa Fluor 568 was added for 5 min. Cells were then washed on ice, fixed and imaged using an epifluorescence microscope. Scale bar = 20 µm. B) Cells were infected with HSV‐1 for 8 h. For the last 30 min cells were incubated with DMSO, 15 µm Dynole or Dynole negative control and 30 µm PitStop2 or its negative control. After 15‐min incubation antibodies specific to viral glycoproteins (LP2 for gD, 3063 for gE, CB24 for gB and LP11 for gH) were added and incubated for a further 15 min. After fixation, cells were permeabilized and stained with secondary antibodies and imaged by epifluorescence microscopy. Scale bar = 20 µm.