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. 2015 Nov 6;17(1):21–39. doi: 10.1111/tra.12340

Figure 4.

TRA-12340-FIG-0004-c

Endocytosis inhibitors impair transport of glycoproteins to cytoplasmic capsids. HFF‐Tert cells were infected with VP26‐mTurquoise/gM‐EYFP recombinant virus for 8 h. Thirty minutes before the end of incubation cells were treated with 15 µm Dynole or Dynole negative control and 30 µm PitStop2 or its negative control. After 15 min antibodies specific to gD (LP2) were added and incubated for a further 15 min. Cells were then fixed, permeabilized and labeled with secondary antibodies. Images were acquired using a confocal microscope. Blue circles indicate complete viral particles while yellow arrows point to particles missing gD staining. Scale bar = 2 µm. Right panels show percentage of intracellular capsids (VP26‐mTurquoise) colocalizing with gD. At least 100 capsids from multiple cells and several fields of view were counted for each condition. Graphs represent the percentage of capsids positive for gD labeling as a mean from two independent experiments ± SEM.