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. 2016 Feb 8;6:20121. doi: 10.1038/srep20121

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Copyright © 2016, Macmillan Publishers Limited

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(A,B) The expression levels of miR-141 (A) and miR-22 (B) were determined by qRT-PCR. These two miRNAs were downregulated during osteogenesis. (C) The hMSCs were tranfected with miR-141 or miR-22 mimics. The hMSC osteogenesis was initiated by osteogenic medium. At day 10 post-induction, the ALP activity was measured and ectopic expression of both miR-141 and miR-22 impaired ALP activity. (D) The osteoblast differentiation of miR-141- or miR-22-overexpressing hMSCs was started by osteogenic medium. At day 20 post-induction, the calcified nodules were visualized by Alizarin Red stainning and it was showed that overexpression of miR-141 or miR-22 inhibited hMSC osteogenesis. Scale bars: 1 cm. (E) The RNA levels of osteogenesis-related marker genes were evaluated by qRT-PCR and ectopic expression of miR-141 or miR-22 downregulated the expression serial osteogenic maker genes. (n = 3; *P < 0.05; **P < 0.01; ***P < 0.001).