Figure 1. Regulation of luciferase expression by rare AGG codon insertion.

(A) Constructs used to generate rare codon devices. Various numbers of rare AGG codons (cyan) were inserted immediately downstream of the start codon of luciferase gene (green) under the influence of the T7 promoter (blue). The cognate tRNA^Arg (CCU) gene (purple) was influenced by the trc promoter (blue). The expression level of the target protein was controlled by the number of rare codon (AGG) insertions. (B) Regulation of luciferase expression using the constructs shown in Fig. 1A. The results observed were fitted to Eq. (1) and are depicted in Fig. 1B (red line).