Figure 5. Modification of the fatty acid synthesis pathway in E. coli via using rare codon devices to regulate expressions of fabZ, fabG, fabI and tesA’.

(A) Constructs of four modified genes in the fatty acid synthetic pathway (4Z8G4I1T) and controls (4Z4G4I4T and ZGIT). The numbers represent the number of rare codons (AGG) inserted immediately after the start codon. (B) Comparison of total fatty acids produced in E. coli containing the modified construct (4Z8G4I1T) and controls (4Z4G4I4T, ZGIT and WT) shown in Fig. 5A. (C) Yields of fatty acids with 14 (C14), 16 (C16) and 18 (C18) carbons were determined and compared among WT E. coli and the strains harboring the plasmids mentioned in 5A.