Figure 1. Chelatable Zn in the PGz of zebrafish brain stained by Neo-Timm after hypoxia followed by normoxic treatment, with or without DEDTC.

(A) Chelatable Zn staining. Images in lower panels represent higher magnification of the area delimited by the square depicted in upper panels. The higher chelatable Zn content is indicated by the presence of the darker granules. (B) Optic densities of periventricular gray zone. OT, optic tectum; PGz, periventricular gray zone. Bars represent 200 μm in upper panels, and 60 μm in lower panels. In (B) the values are presented as the mean ± SEM (n = 3–4 per group). Different letters indicate statistical differences between groups at p < 0.05 level (two-way ANOVA followed by Tukey’s post hoc test).