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. 2016 Feb 8;6:20431. doi: 10.1038/srep20431

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The diagram illustrates that a PCR product will only be present if recircularization of the replicon occurred. LIR, long intergenic region; arrows indicate primer positions. The bottom right part of the picture indicate the PCR products resulting from the inverse PCR; L, GeneRuler 1 kb DNA Ladder (Life Technologies); 1, mSEAP fraction 8 with proteinase K; 2 mSEAP fraction 8 without proteinase K; 3, mSEAP fraction 13 with proteinase K; 4, mSEAP fraction 13 without proteinase K.