Table 1. Genomic DNA analysis of integration sites in 13 selected PTS (phototaxis-screening) mutants.
| Mutant | Location | Protein IDb | Functionc | Methods for sequence determination |
|---|---|---|---|---|
| PTS1 | promoter (B)a | 516748 | Signal recognition particle receptor | TAIL-PCR & PCR-SPd |
| PTS23 | promoter (B) | 525919 | Similar to homeobox A3 (Mus musculus) | Inverse PCR & PCR-SP |
| PTS26 | 17th intron (B) | 516786 | Ionotropic glutamate receptor | TAIL-PCR & PCR-SP |
| PTS36 | 5′-UTR (B) | 519251 | Putative glycine-rich cell wall structural protein | TAIL-PCR & PCR-SP |
| PTS37 | 5′-UTR (B) | 519327 | Glycoside hydrolase | TAIL-PCR & PCR-SP |
| PTS42 | promoter (B) | 516641 | Protein binding; PHD-type zinc finger | TAIL-PCR & PCR-SP |
| PTS59 | 3rd intron (S) | 523869 | Diacylglycerol acyltransferase family | TAIL-PCR |
| PTS61 | 3′-UTR (B) | 513005 | Vesicle-mediated transport; t-SNARE family | TAIL-PCR & PCR-SP |
| PTS64 | 2nd intron (B) | 520695 | Biopterin transport-related protein BT1 | TAIL-PCR & PCR-SP |
| PTS66 | 3′-UTR (B) | 518826 | Flagellar associated protein | TAIL-PCR & PCR-SP |
| PTS69 | 5′-UTR (S) | 515661 | 3-phosphoshikimate 1-carboxyvinyltransferase | TAIL-PCR |
| PTS118 | 5′-UTR (B) | 513996 | Nucleosome assembly; Histone H4 | TAIL-PCR & PCR-SP |
| PTS124 | 6th exon (B) | 512634 | GDP mannose transporter | TAIL-PCR & PCR-SP |
aB: both flanking sequences were identified, S: single flanking sequence was identified.
bProtein IDs were obtained from JGI Chlamydomonas v4.0 (encoded by gene closest to the insertion site).
cFunction (domain, motif) was described based on the JGI Chlamydomonas v4.0.
dPCR-SP: PCR using specific primers (Supplementary Table S1).