Table 1. Preparation of gene-targeted cell clones.
| Cell lines | Drug-resistant cell clones | 3′-junction positive cell clones (%) | 5′-junction positive cell clones (%) | LR-PCR positive cell clones (%) | Senescent cell clones* | Cell clones suitable for SCNT** |
|---|---|---|---|---|---|---|
| BFF10-20 | 317 | 27 (8.5) | 21 (6.6) | 18 (5.7) | 5 | 13 |
| BFF11-21 | 235 | 19 (8.1) | 15 (6.4) | 13 (5.5) | 5 | 8 |
| BFF07-23 | 283 | 15 (5.3) | 12 (4.3) | 9 (3.2) | 4 | 5 |
| Total | 835 | 61 (7.3) | 48 (5.7) | 40 (4.8) | 14 | 26 |
*No cell proliferation was observed in a week.
**The chromosome number of targeted cells was normal.