Table 1.
Primers and expected sizes of PCR products with each primer pair.
| Gene | Primer sequence (5′→3′) | Product size (bp) | GenBank accession number |
|---|---|---|---|
| PPARγ | Forward: GACCACTCGCATTCCTTT | 459 | NM_001127330.1 |
| Reverse: GGCATTGTGAGACATCCC | |||
|
| |||
| C/EBPα | Forward: CTATCAGTCTGTCCAGCCC | 302 | NM_007678.3 |
| Reverse: CTATCAGTCTGTCCAGCCC | |||
|
| |||
| SREBP-1c | Forward: AAGGGCCAGGAGTGGGTAAAC | 226 | NM_011480.3 |
| Reverse: CGCGCCGCGCCCCATTAGG | |||
|
| |||
| FAS | Forward: CAGTTCCAGCCATGAAGAGA | 192 | NM_007988.3 |
| Reverse: TTTGCTGGCAAAGAGAACAC | |||
|
| |||
| ACC | Forward: TCTATTCGGGGTGACTTTC | 350 | NM_133360.2 |
| Reverse: CTATCAGTCTGTCCAGCCC | |||
|
| |||
| GAPDH | Forward: AGATCCACAACGGATACATT | 309 | NM_008084 |
| Reverse: TCCCTCAAGATTGTCAGCAA | |||
PPARγ: peroxisome proliferator-activated receptor γ, C/EBPα: CCAAT/enhancer binding protein α, SREBP-1c: sterol regulatory element binding protein 1c, FAS: fatty acid synthase, ACC: acetyl-coenzyme A, and GAPDH: glyceraldehyde-3-phosphate dehydrogenase.